<?xml version="1.0" encoding="US-ASCII"?>
<!-- Generated by Broadridge Transform (tm) - http://www.broadridge.com -->
<!-- Created: Mon Apr 27 20:30:20 UTC 2026 -->
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
  <roleRef xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#IndividualsOnly" roleURI="http://xbrl.sec.gov/ecd/role/IndividualsOnly"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all"/>
  <definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="extended">
    <loc xlink:type="locator" xlink:label="ecd_AllIndividualsMember" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#ecd_AllIndividualsMember"/>
    <loc xlink:type="locator" xlink:label="gyre_PingZhangMember" xlink:href="gyre-20260610.xsd#gyre_PingZhangMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember" xlink:to="gyre_PingZhangMember" order="2" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="gyre_HanYingPhDMember" xlink:href="gyre-20260610.xsd#gyre_HanYingPhDMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember" xlink:to="gyre_HanYingPhDMember" order="4" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="gyre_CharlesWuPhDMember" xlink:href="gyre-20260610.xsd#gyre_CharlesWuPhDMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember" xlink:to="gyre_CharlesWuPhDMember" order="6" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="gyre_SongjiangMaMember" xlink:href="gyre-20260610.xsd#gyre_SongjiangMaMember"/>
<!-- [WMV7][908HPaV5gL5YyagT3DadrcggRFFWmue8Gm+I5WwnK6w+k6Jv6yp9KZHtlMrqMZVbtPsveTIl9BlPC0cIjwo3GNDGxIKidkBz0o7PWnDHExeQROuoQxp2Asx3LlbZFmdueTJVvLPlTd7sFzmeiqOy09b/Z/xm1u/yu28OJJdjx5g/k0JZWYlaHLgpL48oAM0ZC/Q/Dd12K7HNdF1YlFNsimatzNBZi/VEWXpmrMKa+BFDmBQnIYIlHMh3cFB8S1jhBlX65F+Ij2SQaHf29M0lfBc3prHRB6HxV4jQTkjhaRms9HTzSrNQ7ofiw/ME+1ig9Xu7bvsLP3YWWSEaSNpJJ6wVrI6S8wd+PPFyT5jTplB2NlJvPnyNrqn/] CSR-->
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember" xlink:to="gyre_SongjiangMaMember" order="8" xbrldt:closed="true"/>
  </definitionLink>
</linkbase>