<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001975493</cik>
        <entityName>Boot64 Magnolia Fund I LP</entityName>
        <issuerAddress>
            <street1>3421 NORTH CAUSEWAY BLVD, SUITE 101</street1>
            <city>METAIRIE</city>
            <stateOrCountry>LA</stateOrCountry>
            <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
            <zipCode>70002</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>504-621-6058</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Partnership</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2023</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>-</firstName>
                <lastName>Boot64 Ventures LLC</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3421 North Causeway Blvd Suite 101</street1>
                <city>Metairie</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70002</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Manager of the General Partner of the Fund</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>-</firstName>
                <lastName>Boot64 GP LLC</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3421 North Causeway Blvd Suite 101</street1>
                <city>Metairie</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70002</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>General Partner of the Fund</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John</firstName>
                <middleName>K.</middleName>
                <lastName>Roberts III</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3421 North Causeway Blvd Suite 101</street1>
                <city>Metairie</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70002</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Managing Partner of the Manager of the General Partner of the Fund</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Mickal</firstName>
                <middleName>P.</middleName>
                <lastName>Adler</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3421 North Causeway Blvd Suite 101</street1>
                <city>Metairie</city>
                <stateOrCountry>LA</stateOrCountry>
                <stateOrCountryDescription>LOUISIANA</stateOrCountryDescription>
                <zipCode>70002</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Managing Partner of the Manager of the General Partner of the Fund</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Venture Capital Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
            <item>3C</item>
            <item>3C.1</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001975493-25-000002</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2023-06-01</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>10000000</totalOfferingAmount>
            <totalAmountSold>10000000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>30</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse>Boot64 GP LLC and its affiliates receive management fees and performance fees, and/or allocations based on capital commitments of limited partners of the Fund.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Boot64 Magnolia Fund I LP</issuerName>
                <signatureName>John K. Roberts III</signatureName>
                <nameOfSigner>John K. Roberts III</nameOfSigner>
                <signatureTitle>Managing Partner of the Manager of the General Partner</signatureTitle>
                <signatureDate>2026-04-27</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>