<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0609</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-04-21</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001799191</issuerCik>
        <issuerName>Oncology Institute, Inc.</issuerName>
        <issuerTradingSymbol>TOI</issuerTradingSymbol>
        <issuerForeignTradingSymbol></issuerForeignTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002069752</rptOwnerCik>
            <rptOwnerName>Langsam Jeffrey</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>18000 STUDEBAKER ROAD, SUITE 800</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>CERRITOS</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>90703</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Clinical Officer</officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <remarks>Pursuant to a Unanimous Written Consent of the Board of Directors of the Issuer dated April 21, 2026, the reporting person was determined to no longer be an officer of the Issuer for purposes of Section 16 of the Securities Exchange Act of 1934, as amended.</remarks>

    <ownerSignature>
        <signatureName>/s/ Mark Hueppelsheuser, Attorney-in-fact for Jeffrey Langsam</signatureName>
        <signatureDate>2026-04-23</signatureDate>
    </ownerSignature>
</ownershipDocument>